Reverse calcium affinity purification of Fab with calcium derivatized hydroxyapatite.
نویسندگان
چکیده
This study introduces the application of calcium-derivatized hydroxyapatite for purification of Fab. Fab binds to native hydroxyapatite but fails to bind to the calcium derivatized form. IgG, Fc, and most other protein contaminants bind to the calcium form. This supports Fab purification by a simple flow-through method that achieves greater than 95% purity from papain digests and mammalian cell culture supernatants. Alternatively, Fab can be concentrated on native hydroxyapatite then eluted selectively by conversion to the calcium-derivatized form.
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ورودعنوان ژورنال:
- Journal of immunological methods
دوره 342 1-2 شماره
صفحات -
تاریخ انتشار 2009